A master file was created off of the same view in two different locations. 

1. Master file one at original/master_file with define.

2. Master file two at new_area/master_file without define.
 

When creating a report, App Studio would always bring back the master file at original/master_file even if the master file at new_area/master_file was selected (defines and formatting had been created in the master file at new_area/master_file that were not in the master file at original/master_file). If you create a new Report using new_area/master_file from the different location you will not see defined. This is expected behavior.

If you have both new and old master file in different locations and you are trying to access the old master file as Report, it is reading the new master file which does not have defined i.e. App Studio always access the master file at original/master_file even when trying to create a report using new_area/master_file 

This is because in App Studio customer had two master files with the same name in different locations.

Hence, there had been confusion in referring the sub directory.
 

Note: If a report was created in "Workspace" on the App server it always used the master file at the location selected.

To resolve this issue, be sure when selecting the data source in App Studio click on the checkbox specified as 'Use qualified path'.

Please refer the below screenshot